Insulin (Total) MBeads Assay

(10-1353-01)

Kit format: 5 x 96 wells or 1 x 384 wells

Measurement range: 23.3–2500 mU/L (Human ≈1.0–108,7 µg/L, Mouse ≈1.49–160 µg/L)

Low sample volume (5µL)

Short and easy protocol, no dilutions

For use in 96 and 384 well plates

Adapted for manual or automatic procedures

If required, a 96 well bio-magnetic plate can be purchased, requesting item 15-1000-01

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Description

The Insulin (Total) MBeads Assay is a high-throughput tool enabling high specificity results from small sample volumes.

Total Insulin Northern Lights® MBeads Assay is intended to be used for the measurement of insulin in conditioned media from perifusion experiments or other in vitro cultures from cells derived from human and mouse specimens. The assay allows the analysis of multiple samples simultaneously, decreasing the cost per data point and the amount of sample required. This enables the analysis of other analytes in volume-limited samples.

Read more: MBeads Brochure

Test characteristics

Species

Human and Mouse

Sample types

Cell media (perifusion samples, conditioned media)

Sample volume

5 µL

Detection limit

12.25 mU/L

Assay range

23.3 - 2500 mU/L

Incubation time

2 h + 10 min

Detection method

Chemiluminescence

specificity

The following cross-reactions have been tested:

C-peptide

≤ 0.02 %

Proinsulin

59 %

Mouse insulin

68 %

Rat insulin

112 %

directions for use & Safety Data Sheet

Directions for Use - 10-1353-01 Version 2.0 Lot.no 33797

Safety Data Sheet - 10-1353-01

For other languages and older versions:

Technical Library

technical notes

For product-specific technical notes see:
TN34-0166 Total Insulin Northern Lights MBeads Assay
TN34-0168 Alternative 10 µL protocol Total Insulin Northern Lights MBeads Assay

For other technical notes see our Technical Library