Total GIP ELISA

(10-1258-01)

Kit format: 1 x 96 wells

Measurement range: 2.7 - 1000 pmol/L

Superior sensitivity

Broad measuring range

Measures both active GIP(1-42) and the inactive isoform GIP(3-42) in total

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Description

The Mercodia Total GIP ELISA provides a method for the quantitative determination of total GIP in human samples.

Mercodia Total GIP ELISA measures active GIP(1-42) to the same extent as its metabolite GIP(3-42). Thus, the rapid degradation of the active GIP(1-42) form is not a problem for the specificity of the assay. Accurate results are obtained even if samples without any stabilizing additives are analyzed. The Mercodia Total GIP ELISA has also no cross-reactivity to known cross-reactants such as GLP-1, Glucagon, Oxyntomodulin, and Glicentin.

Want to read more about our Total GIP ELISA, see our Characterization of Total GIP NL-ELISA Reagents Report.

Test characteristics

Species

Human

Sample types

Serum and EDTA plasma

Sample volume

25µL

Assay range

2.7 - 1000 pmol/L

Detection limit

≤1.62 pmol/L

Incubation

18-22 h (overnight) + 10 min + 15 min

Detection method

Chemiluminescence

specificity

The following cross-reactions have been tested:

GIP (1-42)

114-124%

GIP (3-42)

100%

GIP (1-30)

Not detected

GIP (3-30)

Not detected

Glucagon

Not detected

GLP-1 (9-36) amide

Not detected

GLP-2

Not detected

Oxyntomodulin

Not detected

Glicentin

Not detected

Mini-glucagon

Not detected

Proglucagon 1-61

Not detected

MPGF

Not detected

PACAP-38

Not detected

directions for use Certificate of Analysis Safety Data Sheet

directions for use

LOT. NO.	DOCUMENT
35949	DfU - 10-1258-01 v. 3.0
35500	DfU - 10-1258-01 v. 3.0
35492	DfU - 10-1258-01 v. 3.0
34167	DfU - 10-1258-01 v. 3.0
34166	DfU - 10-1258-01 v. 3.0

Certificate of Analysis

LOT. NO.	DOCUMENT
35949	CoA - 10-1258-01
35500	CoA - 10-1258-01
35492	CoA - 10-1258-01
34167	CoA - 10-1258-01
34166	CoA - 10-1258-01

Safety Data Sheet

DOCUMENT
SDS - 10-1258-01

For other languages and previous versions: Technical Library

references

Meessen, E. C. E. et. al. (2021). Parenteral nutrition impairs plasma bile acid and gut hormone responses to mixed meal testing in lean healthy men. Clinical Nutrition, 40(3). Read more >

Mose, M. et. al. (2021). β-Lactoglobulin Is Insulinotropic Compared with Casein and Whey Protein Ingestion during Catabolic Conditions in Men in a Double-Blinded Randomized Crossover Trial. The Journal of Nutrition Nutrition and Disease. Read more >

Vestergaard, E. T. et al. (2021). Acute ketosis inhibits appetite and decreases plasma concentrations of acyl ghrelin in healthy young men. Diabetes, Obesity and Metabolism, 23(8), 1834–1842. Read more >

Hummel, J et. al. (2021). Free fatty acids, glicentin and glucose-dependent insulinotropic polypeptide as potential major determinants of fasting substrate oxidation. Scientific Reports 2021 11:1, 11(1), 1–9. Read more >

Pedersen, M. G. B. et. al. (2021). Oral lactate slows gastric emptying and suppresses appetite in young males. Clinical Nutrition. Read more >