C-peptide ELISA

C-peptide ELISA

C-peptide ELISA

(10-1136-01)

Kit format: 1 x 96 wells

Measurement range: 100 - 4000 pmol/L (0.302 - 12.1 µg/L)

No or minimal cross-reactivity to human insulin and proinsulin

High specificity enables measurement of human C-peptide in mouse and rat models

CE/IVD labeled on select markets

Serum controls available: 10-1134-01 (US only) or 10-1164-01

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Description

The C-peptide ELISA is a high quality immunoassay for specific quantification of human C-peptide in serum, plasma or urine

The Mercodia C-peptide ELISA is a highly specific tool registered for in vitro diagnostics (IVD) use. It is calibrated against the International Reference Reagent for human C-peptide, IRR84/510. The specificity of the utilized antibodies enables measurement of human C-peptide without interference from insulin or proinsulin, and measurement of human C-peptide in mouse and rat models.

 

Each Mercodia C-peptide ELISA kit contains reagents for 96 wells, sufficient for 42 samples and one calibrator curve in duplicate. The microplate strip format (12 strips x 8 wells) is ideal when only a limited number of samples need to be analyzed.

 

Mercodia Diabetes Antigen Control (10-1134-01 (US only) or 10-1164-01) is designed to be used as a two-level control for human C-peptide.

Test characteristics
Species

Human

Samples

Serum, EDTA and heparin plasma or urine

Sample volume

25 µL

Assay range

100-4000 pmol/L (0.3-12.0 ng/mL)

Detection limit

≤ 25 pmol/L (0.076 μg/L)

Incubation

1h + 1h + 15 min

Detection method

Absorbance

specificity

The following cross-reactions have been tested:

Insulin

< 0.0006 %

Proinsulin

2 %

Proinsulin (Des31-32)

3 %

Proinsulin (Split 32-33)

2 %

Proinsulin (Des 64-65)

74 %

Proinsulin (Split 65-66)

10 %

directions for use Certificate of Analysis Safety Data Sheet
directions for use
LOT. NO. DOCUMENT
35992 DfU - 10-1136-01 v. 16.0
35702 DfU - 10-1136-01 v. 16.0
35515 DfU - 10-1136-01 v. 16.0
Certificate of Analysis
LOT. NO. DOCUMENT
35992 CoA - 10-1136-01
35702 CoA - 10-1136-01
35515 CoA - 10-1136-01
Safety Data Sheet
DOCUMENT
SDS - 10-1136-01

For other languages and previous versions: Technical Library

references

Tatovic, D. et al. (2016) Stimulated urine C-peptide creatinine ratio vs serum C-peptide level for monitoring of β-cell function in the first year after diagnosis of Type 1 diabetes. Diabet. Med. 33, 1564–1568. Read more >

Honsek, C. et al. (2018) Fibre supplementation for the prevention of type 2 diabetes and improvement of glucose metabolism: the randomised controlled Optimal Fibre Trial (OptiFiT). Diabetologia 61, 1295–1305. Read more >

Kondo, Y. et al. (2017) Identification of a small molecule that facilitates the differentiation of human iPSCs/ESCs and mouse embryonic pancreatic explants into pancreaticendocrine cells. Diabetologia 60, 1454–1466. Read more >

Li, J. et al. (2019) Evaluation of [68Ga]DO3A-VS-Cys40-Exendin-4 as a PET Probe forImaging Human Transplanted Islets in the Liver. Sci. Rep. 9, 5705. Read more >

Question about the product? Contact us

Andreas Almlén

Key Account Manager Distributors and OEM

(+46) 70 780 95 09 BOOK A MEETING
C-peptide ELISA

C-peptide ELISA

(10-1136-01)

ASK A QUESTION OR BOOK A MEETING?
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Andreas Almlén

Key Account Manager Distributors and OEM

(+46) 70 780 95 09 BOOK A MEETING