Ultrasensitive C-peptide ELISA
Ultrasensitive C-peptide ELISA
(10-1141-01)
Kit format: 1 x 96 wells
Measurement range: 5 - 280 pmol/L (0.015 – 0.85 ng/mL)
Very low detection limit < 2.5 pmol/L (0.0076 µg/L)
No or minimal cross-reactivity to insulin or proinsulin
High specificity enables measurement of human C-peptide in mouse and rat models
CE/IVD labeled on select markets
Serum controls available: 10-1134-01 (US only) or 10-1164-01
The Ultrasensitive Mercodia C-peptide ELISA is a high-quality immunoassay for specific quantification of low levels of human C-peptide in serum, plasma, or urine.
The Ultrasensitive Mercodia C-peptide ELISA is a highly specific tool registered for in vitro diagnostics (IVD) use. It is calibrated against the International Reference Reagent for human C-peptide, IRR84/510. The specificity of the utilized antibodies enables measurement of human C-peptide without interference from insulin or proinsulin, and measurement of human C-peptide in mouse and rat models.
Each Mercodia C-peptide ELISA kit contains reagents for 96 wells, sufficient for 42 samples and one calibrator curve in duplicate. The microplate strip format (12 strips x 8 wells) is ideal when only a limited number of samples need to be analyzed.
Higher sensitivity can be achieved by using an alternative protocol described in technical note TN34-0144.
Mercodia Diabetes Antigen Control (10-1134-01 (US only) or 10-1164-01) is designed to be used as a two-level control in the Ultrasensitive C-peptide ELISA.
Human
Serum, EDTA and heparin plasma or urine
50 µL
5 - 280 pmol/L (0.015 – 0.85 ng/mL)
≤2.5 pmol/L (0.0076 µg/L)
1h + 1h + 30 min
Absorbance
The following cross-reactions have been tested:
< 0.0006 %
5 %
3 %
2 %
74 %
10 %
| LOT. NO. | DOCUMENT |
|---|---|
| 36957 | DfU - 10-1141-01 v. 17.0 |
| 36480 | DfU - 10-1141-01 v. 17.0 |
| 36225 | DfU - 10-1141-01 v. 17.0 |
| 36203 | DfU - 10-1141-01 v. 17.0 |
| 35793 | DfU - 10-1141-01 v. 17.0 |
| 35557 | DfU - 10-1141-01 v. 17.0 |
| 35250 | DfU - 10-1141-01 v. 17.0 |
| LOT. NO. | DOCUMENT |
|---|---|
| 36957 | CoA - 10-1141-01 |
| 36480 | CoA - 10-1141-01 |
| 36225 | CoA - 10-1141-01 |
| 36203 | CoA - 10-1141-01 |
| 35793 | CoA - 10-1141-01 |
| 35557 | CoA - 10-1141-01 |
| 35250 | CoA - 10-1141-01 |
For other languages and previous versions: Technical Library
Wang, L., Lovejoy, N. F. & Faustman, D. L. (2012) Persistence of Prolonged C-peptide Production in Type 1 Diabetes as Measured With an Ultrasensitive C-peptide Assay. Diabetes Care 35, 465–470. Read more >
Sackett, S. D. et al. (2018) Extracellular matrix scaffold and hydrogel derived from decellularized and delipidized human pancreas. Sci. Rep. 8, 10452. Read more >
Furuyama, K. et al. (2019) Diabetes relief in mice by glucose-sensing insulin-secreting human α-cells. Nature 567, 43–48. Read more >
Luk, A. O. Y. et al. (2019) Diabetes-related complications and mortality in patients with young-onset latent autoimmune diabetes: A 14-year analysis of the prospective Hong Kong Diabetes register. Diabetes Care 42, 1042–1050. Read more >
Question about the product? Contact us
Customer Success Manager & Area Sales Manager Nordics
robert.almstedt@mercodia.com (+46) 72 292 15 08