Ultrasensitive C-peptide ELISA

Ultrasensitive C-peptide ELISA

(10-1141-01)

Kit format: 1 x 96 wells

Measurement range: 5 - 280 pmol/L (0.015 – 0.85 ng/mL)

High sensitivity, detetction limit < 2.5 pmol/L (0.0076 µg/L)

No or minimal cross-reactivity to insulin or proinsulin

High specificity enables measurement of human C-peptide in mouse and rat models

Calibrated against International Reference Reagent IRR84/510 for human C-peptide

CE/IVD labeled on select markets

Controls available: 10-1134-01 (US only) or 10-1164-01

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Description

The Mercodia Ultrasensitive C-peptide ELISA is a high quality immunoassay for specific quantification of human C-peptide in serum, plasma, cell culture media or urine.

Even higher sensitivity can be achieved by using an alternative protocol described in TechNote 0144.

Test characteristics
Species

Human

Samples

Serum, EDTA and heparin plasma or urine

Sample volume

50 µL

Assay range

5 - 280 pmol/L (0.015 – 0.85 ng/mL)

Detection limit

≤2.5 pmol/L (0.0076 µg/L)

Incubation

1h + 1h + 30 min

Detection method

Absorbance

specificity

The following cross-reactions have been tested:

Insulin

< 0.0006 %

Proinsulin

5 %

Proinsulin (Des 31-32)

3 %

Proinsulin (Split 32-33)

2 %

Proinsulin (Des 64-65)

74 %

Proinsulin (Split 65-66)

10 %

directions for use & Safety Data Sheet

For other languages and older versions:

Technical Library
references

Wang, L., Lovejoy, N. F. & Faustman, D. L. (2012) Persistence of Prolonged C-peptide Production in Type 1 Diabetes as Measured With an Ultrasensitive C-peptide Assay. Diabetes Care 35, 465–470. Read more >

Sackett, S. D. et al. (2018) Extracellular matrix scaffold and hydrogel derived from decellularized and delipidized human pancreas. Sci. Rep. 8, 10452. Read more >

Furuyama, K. et al. (2019) Diabetes relief in mice by glucose-sensing insulin-secreting human α-cells. Nature 567, 43–48. Read more >

Luk, A. O. Y. et al. (2019) Diabetes-related complications and mortality in patients with young-onset latent autoimmune diabetes: A 14-year analysis of the prospective Hong Kong Diabetes register. Diabetes Care 42, 1042–1050. Read more >

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Andreas Almlén

Key Account Manager Distributors and OEM

(+46) 70 780 95 09 BOOK A MEETING

Ultrasensitive C-peptide ELISA

(10-1141-01)

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Andreas Almlén

Key Account Manager Distributors and OEM

(+46) 70 780 95 09 BOOK A MEETING