Glicentin NL-ELISA

(10-1273-01)

Kit format: 1 x 96 wells

Measurement range: 3 – 300 pmol/L (24 – 2400 pg/mL)

Highly specific, sensitive and rapid method for determination of glicentin

No cross-reactivity to circulating proglucagon derived gut peptides

Assay buffers and calibrators have been optimized to prevent degradation and aggregation of glicentin

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Description

The Mercodia Glicentin ELISA is a high quality immunoassay for the quantification of human glicentin in serum, plasma and cell culture media.

Glicentin is a proglucagon derived gut peptide that is released in the gut in response to feed intake. It is present in high levels in the circulation after stimulation and is often a problem when measuring glucagon and other proglucagon derivates because of it’s shared sequence. The Mercodia Glicentin ELISA however, has no cross-reactivity to such circulating proglucagon derived gut peptides.

Test characteristics

Species

Human

Sample types

Serum, EDTA plasma and cell culture media

Sample volume

25 µL

Assay range

3 - 300 pmol/L (24-2400 pg/mL)

Detection limit

≤3 pmol/L (24 pg/mL)

Incubation

1 h + 1 h + 15 min

Detection method

Absorbance

specificity

The following cross-reactions have been tested:

Glucagon

Not detected

Oxyntomodulin

Not detected

Mini-glucagon

Not detected

GLP-1

Not detected

GLP-2

Not detected

Rat glicentin

0.9 %

Mouse glicentin

Not detected

directions for use & Safety Data Sheet

Directions for Use - 10-1273-01 Version 5.0 Lot.no 16897

Safety Data Sheet - 10-1273-01

For other languages and older versions:

Technical Library

references

Manell, H. et al. (2016) Altered Plasma Levels of Glucagon, GLP-1 and Glicentin During OGTT in Adolescents With Obesity and Type 2 Diabetes. J Clin Endocrinol Metab 101, 1181–1189. Read more >

Raffort, J. et al. (2017) Fasting Circulating Glicentin Increases After Bariatric Surgery.Obes. Surg. 27, 1581–1588. Read more >

Poitou, C. et al. (2018) Fasting levels of glicentin are higher in Roux-en-Y gastric bypass patients exhibiting postprandial hypoglycemia during a meal test. Surg. Obes.Relat. Dis. 14, 929–935. Read more >

Roberts, G. P. et al. (2018) Gastrectomy with Roux-en-Y reconstruction as a lean model of bariatric surgery. Surg. Obes. Relat. Dis. 14, 562–568. Read more >