Rat Insulin ELISA

Rat Insulin ELISA

(10-1250-01)

Kit format: 1 x 96 wells

Measurement range: 0.15 - 5.5 μg/L

Low sample volume - 10 μL (5 µL assay protocol available)

Insignificant or no cross reactivity to C-peptide or proinsulin

No interference from complex sample matrices

Controls available: 10-1220-01

TALK TO A PERSON
Description

The Rat Insulin ELISA is based on highly specific monoclonal antibodies with insignificant or no cross-reactivity to C-peptide or proinsulin.

Test characteristics
Species

Rat

Sample types

Serum, EDTA plasma and cell culture medium.

Sample volume

10 µL (5 µL protocol available)

Assay range

0.15 - 5.5 μg/L

Detection limit

≤0.15 µg/L

Incubation

2 h + 15 min

specificity

The following cross-reactions have been tested:

Rat C-peptide

<0.001%

Rat proinsulin

7%

Mouse insulin

75%

Human insulin

167%

Human proinsulin

75%

Human C-peptide

<0.05%

Insulin lispro

167%

directions for use & Safety Data Sheet

For other languages and older versions:

Technical Library
references

Henaoui, I. S., Jacovetti, C., Guerra Mollet, I., Guay, C., Sobel, J., Eliasson, L., & Regazzi, R. (2017). PIWI-interacting RNAs as novel regulators of pancreatic beta cell function. Diabetologia, 60(10), 1977–1986. Read more >

Perry, R. J. et al. (2018). Mechanisms by which a Very-Low-Calorie Diet Reverses Hyperglycemia in a Rat Model of Type 2 Diabetes. Cell Metabolism, 27(1), 210-217.e3. Read more >

Ghiasi, S. M. et al. (2019). Endoplasmic reticulum chaperone glucose-regulated protein 94 is essential for proinsulin handling. Diabetes, 68(4), 747–760. Read more >

Jesinkey, S. R. et al. (2019). Mitochondrial GTP Links Nutrient Sensing to β Cell Health, Mitochondrial Morphology, and Insulin Secretion Independent of OxPhos. Cell Reports, 28(3), 759-772.e10. Read more >

Haythorne, E. et al. (2019). Diabetes causes marked inhibition of mitochondrial metabolism in pancreatic β-cells. Nature Communications, 10(1), 2474. Read more >

Question about the product? Contact us

Andreas Almlén

Key Account Manager Distributors and OEM

(+46) 70 780 95 09 BOOK A MEETING

Rat Insulin ELISA

(10-1250-01)

"*" indicates required fields

Consent*
This field is for validation purposes and should be left unchanged.

ASK A QUESTION OR BOOK A METTING?
WE WILL HELP YOU

Andreas Almlén

Key Account Manager Distributors and OEM

(+46) 70 780 95 09 BOOK A MEETING