Rat Insulin ELISA - 10 pack

Rat Insulin ELISA – 10 pack

(10-1250-10)

Kit format: 10 x 96 wells

Measurement range: 0.15 - 5.5 μg/L

Bulk volumes suitable for large quantities of samples.

Low sample volume - 10 μL (5 µL assay protocol available)

Insignificant or no cross reactivity to C-peptide or proinsulin

Controls available: 10-1220-01

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Description

Mercodia Rat Insulin ELISA – 10 pack

An easy-to-use assay based on highly specific monoclonal antibodies with insignificant or no cross-reactivity to C-peptide or proinsulin. Please note that the 10 pack Mercodia Mouse Insulin ELISA is delivered in bulk and is suitable if you run larger quantities of samples.

Mercodia Diabetes Antigen Control – Rat & Mouse (10-1220-01) is designed to be used as a three-level control for the Mercodia Rat Insulin ELISA.

Test characteristics

Species

Rat

Sample types

Serum, EDTA plasma and cell culture medium.

Sample volume

10 µL (5 µL protocol available)

Assay range

0.15 - 5.5 μg/L

Detection limit

≤0.15 µg/L

Incubation

2 h + 15 min

specificity

The following cross-reactions have been tested:

Rat C-peptide

<0.001%

Rat proinsulin

Mouse insulin

75%

Human insulin

167%

Human proinsulin

75%

Human C-peptide

<0.05%

Insulin lispro

167%

directions for use Certificate of Analysis

directions for use

LOT. NO.	DOCUMENT
36332	DfU - 10-1250-10 v.8.0
33554	DfU - 10-1250-10 v. 7.0
35078	DfU - 10-1250-10 v. 7.0
35582	DfU - 10-1250-10 v. 7.0

Certificate of Analysis

LOT. NO.	DOCUMENT
36332	CoA - 10-1250-10
33554	CoA - 10-1250-01
35078	CoA - 10-1250-01
35582	CoA - 10-1250-01

SAFETY DATA SHEET

technical notes

For product-specific technical notes see:
TN34-0130 5µL sample volume in Mercodia’s Mouse Insulin and Rat Insulin ELISAs

references

Henaoui, I. S., Jacovetti, C., Guerra Mollet, I., Guay, C., Sobel, J., Eliasson, L., & Regazzi, R. (2017). PIWI-interacting RNAs as novel regulators of pancreatic beta cell function. Diabetologia, 60(10), 1977–1986. Read more >

Perry, R. J. et al. (2018). Mechanisms by which a Very-Low-Calorie Diet Reverses Hyperglycemia in a Rat Model of Type 2 Diabetes. Cell Metabolism, 27(1), 210-217.e3. Read more >

Ghiasi, S. M. et al. (2019). Endoplasmic reticulum chaperone glucose-regulated protein 94 is essential for proinsulin handling. Diabetes, 68(4), 747–760. Read more >

Jesinkey, S. R. et al. (2019). Mitochondrial GTP Links Nutrient Sensing to β Cell Health, Mitochondrial Morphology, and Insulin Secretion Independent of OxPhos. Cell Reports, 28(3), 759-772.e10. Read more >

Haythorne, E. et al. (2019). Diabetes causes marked inhibition of mitochondrial metabolism in pancreatic β-cells. Nature Communications, 10(1), 2474. Read more >