Mouse Insulin ELISA

(10-1247-01)

Kit format: 1 x 96 wells

Measurement range: 0.2 - 6.5 µg/L

Low sample volume - 10µL (5µL assay protocol available)

Easy assay protocol

Insignificant or no cross-reactivity to mouse C-peptide or proinsulin

Controls available: 10-1220-01

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Description

The Mercodia Mouse Insulin ELISA is an easy-to-use assay based on highly specific monoclonal antibodies with insignificant or no cross-reactivity to C-peptide or proinsulin.

Test characteristics

Species

Mouse

Sample type

Serum, EDTA plasma and cell culture medium.

Sample volume

10 µL (5 µL protocol available)

Assay range

0.2 - 6.5 µg/L

Detection limit

≤ 0.2 µg/L

Incubation

2 h + 15 min

specificity

The following cross-reactions have been tested:

Mouse C-peptide I

Not detected

Mouse C-peptide II

Not detected

Rat C-peptide I

Not detected

Rat C-peptide II

Not detected

Rat insulin

146 %

Mouse proinsulin I

43 %

Mouse proinsulin II

60 %

Rat proinsulin I

14 %

Rat proinsulin II

60 %

Human insulin

195 %

Human proinsulin

82 %

Human C-peptide

Not detected

directions for use & Safety Data Sheet

Directions for Use - 10-1247-01 Version 9.0 Lot.no 35393

Safety Data Sheet - 10-1247-01

For other languages and older versions:

Technical Library

technical notes

For product-specific technical notes see:
TN34-0130 5µL sample volume in Mercodia’s Mouse Insulin and Rat Insulin ELISAs

references

Fischer, C. et al. (2017). A miR-327-FGF10-FGFR2-mediated autocrine signaling mechanism controls white fat browning. Nature Communications, 8(1), 2079. Read more >

Kleiner, S. et al. (2018). Mice harboring the human SLC30A8 R138X loss-of-function mutation have increased insulin secretory capacity. Proceedings of the National Academy of Sciences of the United States of America, 115(32), E7642–E7649. Read more >

Ikeda, Y. et al. (2018). Glucagon and/or IGF-1 Production Regulates Resetting of the Liver Circadian Clock in Response to a Protein or Amino Acid-only Diet. EBioMedicine, pp. 210–224. Read more >

Figueiredo, H. et al. (2019). Targeting pancreatic islet PTP1B improves islet graft revascularization and transplant outcomes. Science Translational Medicine, 11(497), eaar6294. Read more >

Aarts, S., Reiche, M., den Toom, M., Gijbels, M., Beckers, L., Gerdes, N., & Lutgens, E. (2019). Depletion of CD40 on CD11c+ cells worsens the metabolic syndrome and ameliorates hepatic inflammation during NASH. Scientific Reports, 9(1), 14702. Read more >