Mouse Insulin ELISA

(10-1247-01)

Kit format: 1 x 96 wells

Measurement range: 0.2 - 6.5 µg/L

Low sample volume - 10µL (5µL assay protocol available)

Easy assay protocol

Insignificant or no cross-reactivity to mouse C-peptide or proinsulin

Controls available: 10-1220-01

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Description

Mercodia Mouse Insulin ELISA

An easy-to-use assay based on highly specific monoclonal antibodies with insignificant or no cross-reactivity to C-peptide or proinsulin.

Mercodia Diabetes Antigen Control – Rat & Mouse (10-1220-01) is designed to be used as a three-level control for the Mercodia Mouse Insulin ELISA.

Test characteristics

Species

Mouse

Sample type

Serum, EDTA plasma and cell culture medium.

Sample volume

10 µL (5 µL protocol available)

Assay range

0.2 - 6.5 µg/L

Detection limit

≤ 0.2 µg/L

Incubation

2 h + 15 min

Detection method

Absorbance

specificity

The following cross-reactions have been tested:

Mouse C-peptide I

Not detected

Mouse C-peptide II

Not detected

Rat C-peptide I

Not detected

Rat C-peptide II

Not detected

Rat insulin

146 %

Mouse proinsulin I

43 %

Mouse proinsulin II

60 %

Rat proinsulin I

14 %

Rat proinsulin II

60 %

Human insulin

195 %

Human proinsulin

82 %

Human C-peptide

Not detected

directions for use Certificate of Analysis

directions for use

LOT. NO.	DOCUMENT
36523	DfU - 10-1247-01 v.10.0
36522	DfU - 10-1247-01 v.10.0
36421	DfU - 10-1247-10 v. 9.0
35601	DfU - 10-1247-01 v. 9.0

Certificate of Analysis

LOT. NO.	DOCUMENT
36523	CoA - 10-1247-01
36522	CoA - 10-1247-01
36421	CoA - 10-1247-01
35601	CoA - 10-1247-01

SAFETY DATA SHEET

technical notes

For product-specific technical notes see:
TN34-0130 5µL sample volume in Mercodia’s Mouse Insulin ELISAs

references

Fischer, C. et al. (2017). A miR-327-FGF10-FGFR2-mediated autocrine signaling mechanism controls white fat browning. Nature Communications, 8(1), 2079. Read more >

Kleiner, S. et al. (2018). Mice harboring the human SLC30A8 R138X loss-of-function mutation have increased insulin secretory capacity. Proceedings of the National Academy of Sciences of the United States of America, 115(32), E7642–E7649. Read more >

Ikeda, Y. et al. (2018). Glucagon and/or IGF-1 Production Regulates Resetting of the Liver Circadian Clock in Response to a Protein or Amino Acid-only Diet. EBioMedicine, pp. 210–224. Read more >

Figueiredo, H. et al. (2019). Targeting pancreatic islet PTP1B improves islet graft revascularization and transplant outcomes. Science Translational Medicine, 11(497), eaar6294. Read more >

Aarts, S., Reiche, M., den Toom, M., Gijbels, M., Beckers, L., Gerdes, N., & Lutgens, E. (2019). Depletion of CD40 on CD11c+ cells worsens the metabolic syndrome and ameliorates hepatic inflammation during NASH. Scientific Reports, 9(1), 14702. Read more >