Iso-Insulin ELISA

Iso-Insulin ELISA

(10-1128-01)

Kit format: 1 x 96 wells

Measurement range: 3 - 100 mU/L (0.13 - 4.35 µg/L)

Measures total insulin

Cross-reactivity with most insulin analogs

Calibrated against 1st International Reference Preparation 66/304 for human insulin.

CE/IVD labeled on select markets

Controls available: 10-1134-01 (US only) or 10-1164-01

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Description

Mercodia Iso-Insulin ELISA provides a method for the quantitative determination of total insulin.

It is a high quality enzyme immunoassay for the quantification of insulin; both endogenous and exogenous. The assay measures common, as well as non-commercially available, insulin analogues and comes with full cross-reactivity data.

Combination of Mercodia Iso-Insulin ELISA with the highly specific Mercodia Insulin ELISA enables calculation of analog concentrations by subtracting endogenous insulin.

Test characteristics
Species

Human

Samples

Serum, plasma, cell culture medium

Sample volume

25 µL

Assay range

3 - 100 mU/L (0.13 - 4.35 µg/L)

Detection limit

≤ 2 mU/L (0.086 µg/L)

Incubation

1 h + 15 min

Detection method

Absorbance

specificity

The following cross-reactions have been tested:

Insulin

100 %

C-peptide

<0.1 %

Proinsulin

54 %

Proinsulin des (31-32)

58 %

Proinsulin split (32-33)

56 %

Proinsulin des (64-65)

66 %

Proinsulin split (65-66)

78 %

Insulin lispro

112 %

Insulin aspart

100 %

Insulin determir

28 %

Insulin glargine

58 %

Insulin glulisine

123 %

IGF-I

< 0.02 %

IGF-II

< 0.02 %

Rat insulin

71 %

Mouse insulin

49 %

Porcine insulin

76 %

Ovine insulin

131 %

Bovine insulin

58 %

directions for use & Safety Data Sheet

For other languages and older versions:

Technical Library
references

Fink, H., Herbert, C., & Gilor, C. (2018). Pharmacodynamics and pharmacokinetics of insulin detemir and insulin glargine 300 U/mL in healthy dogs. Domestic Animal Endocrinology, 64, 17–30. Read more >

Regittnig, W. et al. (2019). Insulin Bolus Administration in Insulin Pump Therapy: Effect of Bolus Delivery Speed on Insulin Absorption from Subcutaneous Tissue. Diabetes Technology & Therapeutics, 21(1), 44–50. Read more >

Wang, J. et al. (2019). Charge-switchable polymeric complex for glucose-responsive insulin delivery in mice and pigs. Science Advances, 5(7), 4357–4367. Read more >

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Andreas Almlén

Key Account Manager Distributors and OEM

(+46) 70 780 95 09 BOOK A MEETING

Iso-Insulin ELISA

(10-1128-01)

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Andreas Almlén

Key Account Manager Distributors and OEM

(+46) 70 780 95 09 BOOK A MEETING