Rat Insulin ELISA

(10-1250-01)

Kit format: 1 x 96 wells

Measurement range: 0.15 - 5.5 μg/L

Low sample volume - 10 μL (5 µL assay protocol available)

Insignificant or no cross reactivity to C-peptide or proinsulin

No interference from complex sample matrices

Controls available: 10-1220-01

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Description

Mercodia Rat Insulin ELISA

An assay based on highly specific monoclonal antibodies with insignificant or no cross-reactivity to C-peptide or proinsulin.

Mercodia Diabetes Antigen Control – Rat & Mouse (10-1220-01) is designed to be used as a three-level control for the Mercodia Rat Insulin ELISA.

Test characteristics

Species

Rat

Sample types

Serum, EDTA plasma and cell culture medium.

Sample volume

10 µL (5 µL protocol available)

Assay range

0.15 - 5.5 μg/L

Detection limit

≤0.15 µg/L

Incubation

2 h + 15 min

Detection method

Absorbance

specificity

The following cross-reactions have been tested:

Rat C-peptide

<0.001%

Rat proinsulin

Mouse insulin

75%

Human insulin

167%

Human proinsulin

75%

Human C-peptide

<0.05%

Insulin lispro

167%

directions for use Certificate of Analysis Safety Data Sheet

directions for use

LOT. NO.	DOCUMENT
35930	DfU - 10-1250-01 v. 7.0
33553	DfU - 10-1250-01 v. 7.0

Certificate of Analysis

LOT. NO.	DOCUMENT
35930	CoA - 10-1250-01
33553	CoA - 10-1250-01

Safety Data Sheet

DOCUMENT
SDS - 10-1250-01

For other languages and previous versions: Technical Library

technical notes

For product-specific technical notes see:
TN34-0130 5µL sample volume in Mercodia’s Mouse Insulin and Rat Insulin ELISAs

references

Henaoui, I. S., Jacovetti, C., Guerra Mollet, I., Guay, C., Sobel, J., Eliasson, L., & Regazzi, R. (2017). PIWI-interacting RNAs as novel regulators of pancreatic beta cell function. Diabetologia, 60(10), 1977–1986. Read more >

Perry, R. J. et al. (2018). Mechanisms by which a Very-Low-Calorie Diet Reverses Hyperglycemia in a Rat Model of Type 2 Diabetes. Cell Metabolism, 27(1), 210-217.e3. Read more >

Ghiasi, S. M. et al. (2019). Endoplasmic reticulum chaperone glucose-regulated protein 94 is essential for proinsulin handling. Diabetes, 68(4), 747–760. Read more >

Jesinkey, S. R. et al. (2019). Mitochondrial GTP Links Nutrient Sensing to β Cell Health, Mitochondrial Morphology, and Insulin Secretion Independent of OxPhos. Cell Reports, 28(3), 759-772.e10. Read more >

Haythorne, E. et al. (2019). Diabetes causes marked inhibition of mitochondrial metabolism in pancreatic β-cells. Nature Communications, 10(1), 2474. Read more >