Ultrasensitive Mouse Insulin ELISA

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Minimal Number of Kits Required:

Ultrasensitive Mouse Insulin ELISA

(10-1249-01)

Kit format: 1 x 96 wells

Measurement range: 0.025 - 1.5 μg/L

Very low detection limit - 0.025 µg/L

High specificity - Insignificant cross-reactivity to C-peptide and proinsulin

No interference from complex sample matrices

Controls available: 10-1220-01

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Description

Mercodia Ultrasensitive Mouse Insulin ELISA

A method for the quantitative determination of low levels of insulin in mouse samples. The Ultrasensitive Mouse Insulin ELISA is based on highly specific monoclonal antibodies with insignificant or no cross-reactivity to C-peptide or proinsulin.

Mercodia Diabetes Antigen Control – Rat & Mouse (10-1220-01) is designed to be used as a three-level control for the Mercodia Ultrasensitive Mouse Insulin ELISA.

Test characteristics

Species

Mouse

Samples types

Serum, EDTA plasma and cell culture medium.

Sample volume

25 µL

Assay range

0.025 - 1.0 μg/L

Detection limit

≤0.025 µg/L

Incubation

2 h + 30 min

Detetction method

Absorbance

specificity

The following cross-reactions have been tested:

Rat insulin

146%

Rat proinsulin

14%

Rat C-peptide

Not detected

Human insulin

195%

Human proinsulin

82%

Human C-peptide

Not detected

directions for use Certificate of Analysis

directions for use

LOT. NO.	DOCUMENT
36723	10-1249-01 DfU v 8.0
36312	10-1249-01 DfU v 8.0
36092	10-1249-01 DfU v 8.0
35600	DfU - 10-1249-01 v. 7.0

Certificate of Analysis

LOT. NO.	DOCUMENT
36723	CoA - 10-1249-01
36312	CoA - 10-1249-01
36092	CoA - 10-1249-01
35600	CoA - 10-1249-01

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references

Chakravarthy, H. et al. (2017). Converting Adult Pancreatic Islet α Cells into β Cells by Targeting Both Dnmt1 and Arx. Cell Metabolism, 25(3), 622–634. Read more >

Hunter, R. W. et al. (2018). Metformin reduces liver glucose production by inhibition of fructose-1-6-bisphosphatase. Nature Medicine, 24(9), 1395–1406. Read more >

Huang, Y. et al. (2019). lncRNA Gm10451 regulates PTIP to facilitate iPSCs-derived β-like cell differentiation by targeting miR-338-3p as a ceRNA. Biomaterials, 216, 119266. Read more >

Gilardi, F. et al. (2019). Systemic PPARγ deletion in mice provokes lipoatrophy, organomegaly, severe type 2 diabetes and metabolic inflexibility. Metabolism: Clinical and Experimental, 95, 8–20. Read more >