Lp(a) ELISA

Kit Calculator

Estimate required kits based on your number of samples.

Samples

Article no.

Sample setup

Add the number of samples you plan to run. Please ensure that each sample has sufficient volume to meet the kit’s per-well requirement.

Controls

Add a corresponding Mercodia control kit. Note: If controls are selected, more than one control kit may be needed depending on the number of assay kits used and the time between runs

The results show the estimated number of assay kits required based on your input and the assay’s DfU. Actual kit usage may vary depending on workflow and run frequency.

Minimal Number of Kits Required:

(10-1106-01)

Kit format: 1 x 96 wells

Measurement range: 0.3-5.0 U/L

Calibrated against a highly purified, fully validated, commercial Lp(a) preparation.

Short incubation time

CE/IVD labeled on selected markets

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Description

Mercodia Lp(a) ELISA provides a method for the quantitative determination of human Lipoprotein (a) in serum or plasma.

The Mercodia Lp(a) ELISA is a solid phase two-site enzyme immunoassay based on the sandwich technique, in which two monoclonal antibodies are directed against separate antigenic determinants on the apolipoprotein(a) molecule. Apolipoprotein(a) in the sample reacts with anti-apolipoprotein(a) antibodies bound to microtitration wells and peroxidase-conjugated anti-apolipoprotein(a) antibodies in the solution.

Test characteristics

Species

Human

Samples

Serum, EDTA and heparin plasma

Sample volume

25 µL

Assay range

0.3 - 5.0 U/L

Detection limit

≤0.07 U/L

Incubation

1 h + 1 h + 15 min

Detection method

Absorbance

specificity

The following cross-reactions have been tested:

Plasminogen

Not detected

Apolipoprotein B

Not detected

directions for use Certificate of Analysis

directions for use

LOT. NO.	DOCUMENT
37166	DfU - 10-1106-01 v.12.0
36721	DfU - 10-1106-01 v.12.0
36372	DfU - 10-1106-01 v.12.0
36141	DfU - 10-1106-01 v.12.0
35610	DfU - 10-1106-01 v.12.0
34008	DfU - 10-1106-01 v.12.0

Certificate of Analysis

LOT. NO.	DOCUMENT
37166	CoA - 10-1106-01
36721	CoA - 10-1106-01
36372	CoA - 10-1106-01
36141	CoA - 10-1106-01
35610	CoA - 10-1106-01
34008	CoA - 10-1106-01

For other languages and previous versions: Technical Library

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references

Enkhmaa, B., Anuurad, E., Zhang, W., Kim, K. & Berglund, L. (2019) Heritability of apolipoprotein (a) traits in two-generational African-American and Caucasian families. J. Lipid Res. 60, 1603–1609. Read more >

Low, H. et al. (2019) HIV disease, metabolic dysfunction and atherosclerosis: A three year prospective study. PLoS One 14, e0215620. Read more >