Lp(a) ELISA

(10-1106-01)

Kit format: 1 x 96 wells

Measurement range: 0.3-5.0 U/L

Calibrated against a highly purified, fully validated, commercial Lp(a) preparation.

Short incubation time

CE/IVD labeled on selected markets

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Description

Mercodia Lp(a) ELISA provides a method for the quantitative determination of human Lipoprotein (a) in serum or plasma.

It is a solid phase two-site enzyme immunoassay based on the sandwich technique, in which two monoclonal antibodies are directed against separate antigenic determinants on the apolipoprotein(a) molecule. Apolipoprotein(a) in the sample reacts with anti-apolipoprotein(a) antibodies bound to microtitration wells and peroxidase-conjugated anti-apolipoprotein(a) antibodies in the solution.

Test characteristics

Species

Human

Samples

Serum, EDTA and heparin plasma

Sample volume

25 µL

Assay range

0.3 - 5.0 U/L

Detection limit

≤0.07 U/L

Incubation

1 h + 1 h + 15 min

Detection method

Absorbance

specificity

The following cross-reactions have been tested:

Plasminogen

Not detected

Apolipoprotein B

Not detected

directions for use & Safety Data Sheet

Directions for Use - 10-1106-01 Version 12.0 Lot.no 32465, 32595, 34008

Safety Data Sheet - 10-1106-01

For other languages and older versions:

Technical Library

references

Enkhmaa, B., Anuurad, E., Zhang, W., Kim, K. & Berglund, L. (2019) Heritability of apolipoprotein (a) traits in two-generational African-American and Caucasian families. J. Lipid Res. 60, 1603–1609. Read more >

Low, H. et al. (2019) HIV disease, metabolic dysfunction and atherosclerosis: A three year prospective study. PLoS One 14, e0215620. Read more >