HNL Homodimer ELISA

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HNL Homodimer ELISA

(10-1380-01)

Kit format: 1 x 96 wells

Measurement range: 0-5 μg/L

Specific measurement of homodimeric HNL

Promising biomarker for sepsis monitoring

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Description

The HNL Homodimer ELISA is immunoassay for specific quantification of human homodimeric HNL in EDTA plasma

Human Neutrophil Lipocalin (HNL) (1), also known as Neutrophil Gelatinase-Associated Lipocalin (NGAL) (2), is a glycoprotein that exists in multiple forms, including a 45-kDa disulfide-linked homodimer. This homodimeric form of HNL is a significant component of the secondary granules in human neutrophil granulocytes and is released into the extracellular environment upon activation of the cells. (1,3)

Studies (4, 5) have demonstrated that plasma levels of the HNL homodimer can effectively monitor the success of antibiotic treatment in sepsis patients, providing faster and more reliable responses compared to traditional biomarkers. This positions the HNL homodimer as a promising future tool, with the potential to significantly enhance patient outcomes by enabling more timely and targeted therapeutic interventions (6). The HNL homodimer ELISA is indeeded for Research Use Only (RUO).

Test characteristics

Species

Human

Sample types

EDTA plasma

Sample volume

5 µL

Assay range

0-5 μg/L

Detection limit

< 0.039 μg/L

Incubation

~3 hrs

Detection method

Absorbance

specificity

The following cross-reactions have been tested:

<0.1% cross reactivity with EPX/EDN, ECP, EPO, Lysozyme, and MPO (data generated by trusted partner)

directions for use Certificate of Analysis

directions for use

LOT. NO.	DOCUMENT
Lj1380-015	DfU - 10-1380-01 v. 1.0

Certificate of Analysis

LOT. NO.	DOCUMENT
Lj1380-015	CoA - 10-1380-01

For other languages and previous versions: Technical Library

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references

1. Xu, S. Y., Carlson, M., Engström, A., Garcia, R., Peterson, C. G., & Venge, P. (1994). Purification and characterization of a human neutrophil lipocalin (HNL) from the secondary granules of human neutrophils. Scand J Clin Lab Invest. Read more >

2. Kjeldsen, L., Johnsen, A. H., Sengeløv, H., & Borregaard, N. (1993). Isolation and primary structure of NGAL, a novel protein associated with human neutrophil gelatinase. J Biol Chem. Read more >

3. Cai, L., Rubin, J., Han, W., Venge, P., & Xu, S. (2010). The origin of multiple molecular forms in urine of HNL/NGAL. Clin J Am Soc Nephrol. Read more >

4. Jonsson, N., Gille-Johnson, P., Martling, C.-R., Xu, S., Venge, P., & Mårtensson, J. (2019). Performance of plasma measurement of neutrophil gelatinase-associated lipocalin as a biomarker of bacterial infections in the intensive care unit. J Crit Care. Read more >

5. Venge, P., Peterson, C., Xu, S., Larsson, A., Johansson, J., & Tydén, J. (2024). HNL Dimer in plasma is a unique and useful biomarker for the monitoring of antibiotic treatment in sepsis. PLoS One. Read more >

6. Venge, P., Xu, S., & Mårtensson, J. (2022). HNL Dimer- A Specific Biomarker of Neutrophil Activity - In Sepsis and Antibiotics Monitoring. A Mini-Review. Archives of Clinical and Biomedical Research. Read more >