High Range Rat Insulin ELISA

(10-1145-01)

Kit format: 1 x 96 wells

Measurement range: 3 - 150 μg/L

No sample dilution for sample with high insulin concentrations

Results within 2.5 hours

Low sample volume - 10 µL

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Description

Mercodia High Range Rat Insulin ELISA

A method for the quantitative determination of high levels of insulin in rat samples. The High Range Rat Insulin ELISA is based on highly specific monoclonal antibodies with insignificant or no cross-reactivity to C-peptide or proinsulin.

Mercodia Diabetes Antigen Control – Rat & Mouse (10-1220-01) is designed to be used as a three-level control for the Mercodia High Range Rat Insulin ELISA.

Test characteristics

Species

Rat

Sample types

Serum, EDTA plasma and cell culture medium.

Sample volume

10 µL

Assay range

3 - 150 μg/L

Detection limit

≤1.5 µg/L

Incubation

2 h + 15 min

specificity

The following cross-reactions have been tested:

Mouse insulin

75%

Rat C-peptide I

Not detected

Rat C-peptide II

Not detected

Mouse C-peptide I

Not detected

Mouse C-peptide II

Not detected

Rat Proinsulin I

Rat Proinsulin II

51%

Mouse Proinsulin I

33%

Mouse Proinsulin II

51%

Human C-peptide

Not detected

Human insulin

167%

Human proinsulin

75%

Insulin lispro (Humalog® Eli Lilly)

167%

directions for use Certificate of Analysis

directions for use

LOT. NO.	DOCUMENT
36488	DfU - 10-1145-01 v.11.0
35773	DfU - 10-1145-01 v.11.0
33636	DfU - 10-1145-01 v.11.0
35328	DfU - 10-1145-01 v.11.0

Certificate of Analysis

LOT. NO.	DOCUMENT
36488	CoA - 10-1145-01
35773	CoA - 10-1145-01
33636	CoA - 10-1145-01
35328	CoA - 10-1145-01

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references

Ferri, G. et al. (2019). Insulin secretory granules labelled with phogrin-fluorescent proteins show alterations in size, mobility and responsiveness to glucose stimulation in living β-cells. Scientific Reports, 9(1), 2890. Read more >

Al-Amily, I. M., Dunér, P., Groop, L., & Salehi, A. (2019). The functional impact of G protein-coupled receptor 142 (Gpr142) on pancreatic β-cell in rodent. Pflugers Archiv European Journal of Physiology, 471(4), 633–645. Read more >

Duin, S., Schütz, K., Ahlfeld, T., Lehmann, S., Lode, A., Ludwig, B., & Gelinsky, M. (2019). 3D Bioprinting of Functional Islets of Langerhans in an Alginate/Methylcellulose Hydrogel Blend. Advanced Healthcare Materials, 8(7), e1801631. Read more >

Zhang, E. et al. (2019). Preserving Insulin Secretion in Diabetes by Inhibiting VDAC1 Overexpression and Surface Translocation in β Cells. Cell Metabolism, 29(1), 64-77.e6. Read more >