High Range Rat Insulin ELISA

(10-1145-01)

Kit format: 1 x 96 wells

Measurement range: 3 - 150 μg/L

No sample dilution for sample with high insulin concentrations

Results within 2.5 hours

Low sample volume - 10 µL

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Description

Mercodia High Range Rat Insulin ELISA provides a method for the quantitative determination of high levels of insulin in rat samples.

Test characteristics

Species

Rat

Sample types

Serum, EDTA plasma and cell culture medium.

Sample volume

10 µL

Assay range

3 - 150 μg/L

Detection limit

≤1.5 µg/L

Incubation

2 h + 15 min

specificity

The following cross-reactions have been tested:

Mouse insulin

75%

Rat C-peptide I

Not detected

Rat C-peptide II

Not detected

Mouse C-peptide I

Not detected

Mouse C-peptide II

Not detected

Rat Proinsulin I

Rat Proinsulin II

51%

Mouse Proinsulin I

33%

Mouse Proinsulin II

51%

Human C-peptide

Not detected

Human insulin

167%

Human proinsulin

75%

Insulin lispro (Humalog® Eli Lilly)

167%

directions for use & Safety Data Sheet

Directions for Use - 10-1145-01 Version 11.0 Lot.no 33636, 35328

Safety Data Sheet - 10-1145-01

For other languages and older versions:

Technical Library

references

Ferri, G. et al. (2019). Insulin secretory granules labelled with phogrin-fluorescent proteins show alterations in size, mobility and responsiveness to glucose stimulation in living β-cells. Scientific Reports, 9(1), 2890. Read more >

Al-Amily, I. M., Dunér, P., Groop, L., & Salehi, A. (2019). The functional impact of G protein-coupled receptor 142 (Gpr142) on pancreatic β-cell in rodent. Pflugers Archiv European Journal of Physiology, 471(4), 633–645. Read more >

Duin, S., Schütz, K., Ahlfeld, T., Lehmann, S., Lode, A., Ludwig, B., & Gelinsky, M. (2019). 3D Bioprinting of Functional Islets of Langerhans in an Alginate/Methylcellulose Hydrogel Blend. Advanced Healthcare Materials, 8(7), e1801631. Read more >

Zhang, E. et al. (2019). Preserving Insulin Secretion in Diabetes by Inhibiting VDAC1 Overexpression and Surface Translocation in β Cells. Cell Metabolism, 29(1), 64-77.e6. Read more >