Glucagon ELISA - 10 Microliter

Glucagon ELISA – 10 Microliter

(10-1281-01)

Kit format: 1 x 96 wells

Measurement range: 2-180 pmol/L (7 - 627 pg/mL)

Low sample volume - 10 µL

No purification of samples

Highly appreciated and often cited assay

Controls available: 10-1286-01

Little or no cross-reactivity to oxyntomodulin, glicentin, mini-glucagon, GLP-1, GLP-2 and GRPP

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Description

Mercodia Glucagon ELISA – 10 μL is a highly specific and sensitive immunoassay for measuring glucagon in serum, EDTA plasma, and cell culture media samples from rat, mouse, and non-human primates.

A revolutionizing ELISA for sensitive and specific determination of glucagon in rat, mouse, and non-human primate (NHP) samples. A research tool advancement that allows science to take the next step.

The highly specific monoclonal antibodies used in this assay enable accurate determination of glucagon with low to no levels of significant cross-reactivity to other circulating pro-glucagon-derived peptides. Under certain conditions like post-bariatric surgery, the concentrations of glicentin have been demonstrated to be elevated to levels that might interfere.

Test characteristics

Species

Rat, mouse and non-human primates (NHP)

Sample types

Serum, EDTA plasma and cell culture medium

Sample volume

10 µL

Assay range

2-180 pmol/L (7 - 627 pg/mL)

Detection limit

≤1.5 pmol/L

Incubation

18-22 h (overnight) + 15 min

Detection method

Absorbance

specificity

The following cross-reactions have been tested:

Mini-glucagon

Not detected

Glicentin, human

1.0 %

Glicentin, mouse

7.0 %

Glicentin, rat

4.0 %

GLP-1 (7-36)

Not detected

GLP-1 (9-36)

Not detected

GLP-2

Not detected

Oxyntomodulin, human/rat/mouse

2.0 %

Oxyntomodulin, bovine/canine/porcine

Not detected

directions for use & Safety Data Sheet

Directions for Use - 10-1281-01 Version 6.0 Lot.no 31015, 32318, 32057, 33181, 33440, 33984, 35106, 35570

Safety Data Sheet - 10-1281-01

For other languages and older versions:

Technical Library

technical notes

For product-specific technical notes see:
TN34-0155 Animal samples in Mercodia Glucagon ELISA – 10 µL (10-1281-01)
TN34-0162 Alternative sequential protocol for increased specificity in Mercodia Glucagon ELISA – 10 µL (10-1281-01)

For other technical notes see our Technical Library

references

Pradhan, G., et al. (2021). β Cell GHS-R Regulates Insulin Secretion and Sensitivity. International journal of molecular sciences, 22(8), 3950. Read more >

Takatani, T., et al. (2021). Insulin receptor substrate 1 (IRS1), but not IRS2, plays a dominant role in regulating pancreatic alpha cell function in mice. The Journal of biological chemistry, 100646. Read more >

Chae, H., et al. (2020). SGLT2 is not expressed in pancreatic α- and β-cells, and its inhibition does not directly affect glucagon and insulin secretion in rodents and humans. Molecular metabolism, 42, 101071. Read more >

Adhikari, B., Khanal, P., & Nielsen, M. O. (2018). Impacts of pre- and postnatal nutrition on glucagon regulation and hepatic signalling in sheep. Journal of Endocrinology, 238(1), 1–12. Read more >

Hunter, R. W. et al. (2018). Metformin reduces liver glucose production by inhibition of fructose-1-6-bisphosphatase. Nature Medicine, 24(9), 1395–1406. Read more >

Chakravarthy, H. et al. (2017). Converting Adult Pancreatic Islet α Cells into β Cells by Targeting Both Dnmt1 and Arx. Cell Metabolism, 25(3), 622–634. Read more >

Wewer Albrechtsen, N. J. et al. (2016). Dynamics of glucagon secretion in mice and rats revealed using a validated sandwich ELISA for small sample volumes. American Journal of Physiology. Endocrinology and Metabolism, 311(2) Read more >