Glucagon ELISA – 10 μL
Glucagon ELISA – 10 μL
(10-1281-01)
Kit format: 1 x 96 wells
Measurement range: 2-180 pmol/L (7 - 627 pg/mL)
Low sample volume - 10 µL
No purification of samples
Highly appreciated and often cited assay
Controls available: 10-1286-01
Little or no cross-reactivity to oxyntomodulin, glicentin, mini-glucagon, GLP-1, GLP-2 and GRPP
Mercodia Glucagon ELISA – 10 μL
A revolutionizing ELISA for sensitive and specific determination of glucagon in serum, EDTA plasma, and cell culture media samples from rat, mouse, and non-human primates (NHP). A research tool advancement that allows science to take the next step.
The highly specific monoclonal antibodies used in Glucagon ELISA – 10 µL enable accurate determination of glucagon with low to no levels of significant cross-reactivity to other circulating pro-glucagon-derived peptides. Under certain conditions like post-bariatric surgery, the concentrations of glicentin have been demonstrated to be elevated to levels that might interfere.
Mercodia Glucagon Control – Low, Medium, High (10-1286-01) is a three-level control for Mercodia Glucagon ELISA -10 µL.
An alternative protocol (TN34-0162) for Glucagon ELISA – 10 µL can be used in situations where exceptionally high levels of glicentin are expected.
Rat, mouse and non-human primates (NHP)
Serum, EDTA plasma and cell culture medium
10 µL
2-180 pmol/L (7 - 627 pg/mL)
≤1.5 pmol/L
18-22 h (overnight) + 15 min
Absorbance
The following cross-reactions have been tested:
Not detected
1.0 %
7.0 %
4.0 %
Not detected
Not detected
Not detected
2.0 %
Not detected
| LOT. NO. | DOCUMENT |
|---|---|
| 36021 | DfU - 10-1281-01 v. 6.0 |
| 35570 | DfU - 10-1281-01 v. 6.0 |
| 31015 | DfU - 10-1281-01 v. 6.0 |
| 33984 | DfU - 10-1281-01 v. 6.0 |
| LOT. NO. | DOCUMENT |
|---|---|
| 36021 | CoA - 10-1281-01 |
| 35570 | CoA - 10-1281-01 |
| 35106 | CoA - 10-1281-01 |
| 33984 | CoA - 10-1281-01 |
| DOCUMENT |
|---|
| SDS - 10-1281-01 |
For other languages and previous versions: Technical Library
For technical notes specific to Mercodia Glucagon ELISA – 10 µL see:
TN34-0155 Animal samples in Mercodia Glucagon ELISA – 10 µL (10-1281-01)
TN34-0162 Alternative sequential protocol for increased specificity in Mercodia Glucagon ELISA – 10 µL (10-1281-01)
For other technical notes see our Technical Library
Pradhan, G., et al. (2021). β Cell GHS-R Regulates Insulin Secretion and Sensitivity. International journal of molecular sciences, 22(8), 3950. Read more >
Takatani, T., et al. (2021). Insulin receptor substrate 1 (IRS1), but not IRS2, plays a dominant role in regulating pancreatic alpha cell function in mice. The Journal of biological chemistry, 100646. Read more >
Chae, H., et al. (2020). SGLT2 is not expressed in pancreatic α- and β-cells, and its inhibition does not directly affect glucagon and insulin secretion in rodents and humans. Molecular metabolism, 42, 101071. Read more >
Adhikari, B., Khanal, P., & Nielsen, M. O. (2018). Impacts of pre- and postnatal nutrition on glucagon regulation and hepatic signalling in sheep. Journal of Endocrinology, 238(1), 1–12. Read more >
Hunter, R. W. et al. (2018). Metformin reduces liver glucose production by inhibition of fructose-1-6-bisphosphatase. Nature Medicine, 24(9), 1395–1406. Read more >
Chakravarthy, H. et al. (2017). Converting Adult Pancreatic Islet α Cells into β Cells by Targeting Both Dnmt1 and Arx. Cell Metabolism, 25(3), 622–634. Read more >
Wewer Albrechtsen, N. J. et al. (2016). Dynamics of glucagon secretion in mice and rats revealed using a validated sandwich ELISA for small sample volumes. American Journal of Physiology. Endocrinology and Metabolism, 311(2) Read more >
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andreas.almlen@mercodia.com (+46) 70 780 95 09 BOOK A MEETING
Glucagon ELISA – 10 μL
(10-1281-01)