Glucagon ELISA – 10 μL

Glucagon ELISA - 10 Microliter

Glucagon ELISA – 10 μL

(10-1281-01)

Kit format: 1 x 96 wells

Measurement range: 2-180 pmol/L (7 - 627 pg/mL)

Low sample volume - 10 µL

No purification of samples

Highly appreciated and often cited assay

Controls available: 10-1286-01

Little or no cross-reactivity to oxyntomodulin, glicentin, mini-glucagon, GLP-1, GLP-2 and GRPP

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Description

Mercodia Glucagon ELISA – 10 μL

A revolutionizing ELISA for sensitive and specific determination of glucagon in serum, EDTA plasma, and cell culture media samples from rat, mouse, and non-human primates (NHP). A research tool advancement that allows science to take the next step.

 

The highly specific monoclonal antibodies used in Glucagon ELISA – 10 µL enable accurate determination of glucagon with low to no levels of significant cross-reactivity to other circulating pro-glucagon-derived peptides. Under certain conditions like post-bariatric surgery, the concentrations of glicentin have been demonstrated to be elevated to levels that might interfere.

 

Mercodia Glucagon Control – Low, Medium, High (10-1286-01) is a three-level control for Mercodia Glucagon ELISA -10 µL.

 

An alternative protocol (TN34-0162) for Glucagon ELISA – 10 µL can be used in situations where exceptionally high levels of glicentin are expected.

Test characteristics
Species

Rat, mouse and non-human primates (NHP)

Sample types

Serum, EDTA plasma and cell culture medium

Sample volume

10 µL

Assay range

2-180 pmol/L (7 - 627 pg/mL)

Detection limit

≤1.5 pmol/L

Incubation

18-22 h (overnight) + 15 min

Detection method

Absorbance

specificity

The following cross-reactions have been tested:

Mini-glucagon

Not detected

Glicentin, human

1.0 %

Glicentin, mouse

7.0 %

Glicentin, rat

4.0 %

GLP-1 (7-36)

Not detected

GLP-1 (9-36)

Not detected

GLP-2

Not detected

Oxyntomodulin, human/rat/mouse

2.0 %

Oxyntomodulin, bovine/canine/porcine

Not detected

directions for use Certificate of Analysis Safety Data Sheet
directions for use
LOT. NO. DOCUMENT
36021 DfU - 10-1281-01 v. 6.0
35570 DfU - 10-1281-01 v. 6.0
31015 DfU - 10-1281-01 v. 6.0
33984 DfU - 10-1281-01 v. 6.0
Certificate of Analysis
LOT. NO. DOCUMENT
36021 CoA - 10-1281-01
35570 CoA - 10-1281-01
35106 CoA - 10-1281-01
33984 CoA - 10-1281-01
Safety Data Sheet
DOCUMENT
SDS - 10-1281-01

For other languages and previous versions: Technical Library

technical notes

For technical notes specific to Mercodia Glucagon ELISA – 10 µL see:
TN34-0155 Animal samples in Mercodia Glucagon ELISA – 10 µL (10-1281-01)
TN34-0162 Alternative sequential protocol for increased specificity in Mercodia Glucagon ELISA – 10 µL (10-1281-01)

 

For other technical notes see our Technical Library

references

Pradhan, G., et al. (2021). β Cell GHS-R Regulates Insulin Secretion and Sensitivity. International journal of molecular sciences, 22(8), 3950. Read more >

Takatani, T., et al. (2021). Insulin receptor substrate 1 (IRS1), but not IRS2, plays a dominant role in regulating pancreatic alpha cell function in mice. The Journal of biological chemistry, 100646. Read more >

Chae, H., et al. (2020). SGLT2 is not expressed in pancreatic α- and β-cells, and its inhibition does not directly affect glucagon and insulin secretion in rodents and humans. Molecular metabolism, 42, 101071. Read more >

Adhikari, B., Khanal, P., & Nielsen, M. O. (2018). Impacts of pre- and postnatal nutrition on glucagon regulation and hepatic signalling in sheep. Journal of Endocrinology, 238(1), 1–12. Read more >

Hunter, R. W. et al. (2018). Metformin reduces liver glucose production by inhibition of fructose-1-6-bisphosphatase. Nature Medicine, 24(9), 1395–1406. Read more >

Chakravarthy, H. et al. (2017). Converting Adult Pancreatic Islet α Cells into β Cells by Targeting Both Dnmt1 and Arx. Cell Metabolism, 25(3), 622–634. Read more >

Wewer Albrechtsen, N. J. et al. (2016). Dynamics of glucagon secretion in mice and rats revealed using a validated sandwich ELISA for small sample volumes. American Journal of Physiology. Endocrinology and Metabolism, 311(2) Read more >

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Andreas Almlén

Key Account Manager Distributors and OEM

(+46) 70 780 95 09 BOOK A MEETING
Glucagon ELISA - 10 Microliter

Glucagon ELISA – 10 μL

(10-1281-01)

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Andreas Almlén

Key Account Manager Distributors and OEM

(+46) 70 780 95 09 BOOK A MEETING