Rat C-Peptide ELISA

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Minimal Number of Kits Required:

Rat C-Peptide ELISA

(10-1172-01)

Kit format: 1 x 96 wells

Measurement range: 100 - 4000 pmol/L (0.33 - 13 µg/L)

Low sample volume - 10μL

Measures both rat C-peptide I and II

Minimal cross-reactivity to human or mouse insulin, C-peptide or proinsulin

Controls available: 10-1220-01

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Description

Mercodia Rat C-peptide ELISA

A method for the quantitative determination of rat C-peptide in serum, EDTA-plasma and cell culture medium.

Mercodia Diabetes Antigen Control – Rat & Mouse (10-1220-01) is designed to be used as a three-level control for the Mercodia Rat C-peptide ELISA.

Test characteristics

Species

Rat

Sample types

Serum, EDTA plasma or cell culture medium

Sample volume

10 µL

Assay range

100 - 4000 pmol/L (0.33 - 13 µg/L)

Detection limit

≤100 pmol/L (0.33 μg/L)

Incubation

1h + 1h + 15 min

Detection method

Absorbance

specificity

The following cross-reactions have been tested:

The ELISA kit measures both Rat C-peptide I and Rat C-peptide II.

Rat insulin

Not detected

Rat proinsulin

4.6 %

Mouse C-peptide

6 %

Porcine C-peptide

Not detected

Human C-peptide

Not detected

directions for use Certificate of Analysis

directions for use

LOT. NO.	DOCUMENT
36732	DfU - 10-1172-01 v.9.0
35774	DfU - 10-1172-01 v.9.0
35316	DfU - 10-1172-01 v.9.0

Certificate of Analysis

LOT. NO.	DOCUMENT
36732	CoA - 10-1172-01
35774	CoA - 10-1172-01
35316	CoA - 10-1172-01

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references

Gu, L. H. et al. (2015) Immunogenicity of allogeneic mesenchymal stem cells transplanted via different routes in diabetic rats. Cell. Mol. Immunol. 12, 444–455. Read more >

Morettini, M. et al. (2017) IVGTT-based simple assessment of glucose tolerance in the Zucker fatty rat: Validation against minimal models. (2018) PLoS One 12, e0173200. Read more >

Holdcraft, R. W., Dumpala, P. R., Smith, B. H. & Gazda, L. S. (2018) A model for determining an effective in vivo dose of transplanted islets based on in vitro insulin secretion. Xenotransplantation 25, e12443. Read more >