Ultrasensitive Rat Insulin ELISA

(10-1251-01)

Kit format: 1 x 96 wells

Measurement range: 0.02 - 1.0 μg/L

Very low detection limit - 0.02 µg/L

High specificity - Insignificant cross-reactivity to C-peptide and proinsulin

No interference from complex sample matrices

Controls available: 10-1220-01

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Description

Mercodia Ultrasensitive Rat Insulin ELISA provides a method for the quantitative determination of low levels of insulin in rat samples.

Test characteristics

Species

Rat

Samples types

Serum, EDTA plasma and cell culture medium.

Sample volume

25 µL

Assay range

0.02 - 1.0 μg/L

Detection limit

≤0.02 µg/L

Incubation

2 h + 30 min

specificity

The following cross-reactions have been tested:

Mouse insulin

75 %

Rat proinsulin I

8 %

Rat proinsulin II

51 %

Mouse proinsulin I

31 %

Mouse proinsulin II

51 %

Mouse c-peptide I

Not detected

Mouse c-peptide II

Not detected

Rat c-peptide I

Not detected

Rat c-peptide II

Not detected

Human insulin

167 %

Human proinsulin

75 %

Human C-peptide

Not detected

Insulin lispro (Humalog®)

167 %

directions for use & Safety Data Sheet

Directions for Use - 10-1251-01 Version 6.0 Lot.no 32776

Safety Data Sheet - 10-1251-01

For other languages and older versions:

Technical Library

references

Singh, H., Ajumeera, R., Malakapalli, V., Chalasani, M., Pothani, S., & Venkatesan, V. (2017). WNIN Mutant Obese Rats Develop Acute Pancreatitis With the Enhanced Inflammatory Milieu. Cellular and Molecular Medicine Research, 1(1), 20–31. Read more >

Ma, D., Ozanne, S. E., & Guest, P. C. (2018). Generation of the maternal low-protein rat model for studies of metabolic disorders. In Methods in Molecular Biology (Vol. 1735, pp. 201–206). Read more >

Lin, P. et al. (2019). Dietary Glycotoxins , Advanced Glycation End Products , Inhibit Cell Proliferation and Progesterone Secretion in Ovarian Granulosa Cells and Mimic PCOS-Like Symptoms. Biomolecules, 9(327), 1–17. Read more >