Ultrasensitive Rat Insulin ELISA

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Minimal Number of Kits Required:

Ultrasensitive Rat Insulin ELISA

(10-1251-01)

Kit format: 1 x 96 wells

Measurement range: 0.02 - 1.0 μg/L

Very low detection limit - 0.02 µg/L

High specificity - Insignificant cross-reactivity to C-peptide and proinsulin

No interference from complex sample matrices

Controls available: 10-1220-01

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Description

Mercodia Ultrasensitive Rat Insulin ELISA

A method for the quantitative determination of low levels of insulin in rat samples. The Rat Insulin ELISA is based on highly specific monoclonal antibodies with insignificant or no cross-reactivity to C-peptide or proinsulin.

Mercodia Diabetes Antigen Control – Rat & Mouse (10-1220-01) is designed to be used as a three-level control for the Mercodia Ultrasensitive Rat Insulin ELISA.

Test characteristics

Species

Rat

Samples types

Serum, EDTA plasma and cell culture medium.

Sample volume

25 µL

Assay range

0.02 - 1.0 μg/L

Detection limit

≤0.02 µg/L

Incubation

2 h + 30 min

Detection method

Absorbance

specificity

The following cross-reactions have been tested:

Mouse insulin

75 %

Rat proinsulin I

8 %

Rat proinsulin II

51 %

Mouse proinsulin I

31 %

Mouse proinsulin II

51 %

Mouse c-peptide I

Not detected

Mouse c-peptide II

Not detected

Rat c-peptide I

Not detected

Rat c-peptide II

Not detected

Human insulin

167 %

Human proinsulin

75 %

Human C-peptide

Not detected

Insulin lispro (Humalog®)

167 %

directions for use Certificate of Analysis

directions for use

LOT. NO.	DOCUMENT
36688	10-1251-01 DfU v 7.0
36244	10-1251-01 DfU v 7.0
35920	10-1251-01 DfU v 7.0
32776	10-1251-01 DfU v. 6.0

Certificate of Analysis

LOT. NO.	DOCUMENT
36688	CoA - 10-1251-01
36244	CoA - 10-1251-01
35920	10-1251-01 CoA 35920
32776	10-1251-01 CoA 32776

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references

Singh, H., Ajumeera, R., Malakapalli, V., Chalasani, M., Pothani, S., & Venkatesan, V. (2017). WNIN Mutant Obese Rats Develop Acute Pancreatitis With the Enhanced Inflammatory Milieu. Cellular and Molecular Medicine Research, 1(1), 20–31. Read more >

Ma, D., Ozanne, S. E., & Guest, P. C. (2018). Generation of the maternal low-protein rat model for studies of metabolic disorders. In Methods in Molecular Biology (Vol. 1735, pp. 201–206). Read more >

Lin, P. et al. (2019). Dietary Glycotoxins , Advanced Glycation End Products , Inhibit Cell Proliferation and Progesterone Secretion in Ovarian Granulosa Cells and Mimic PCOS-Like Symptoms. Biomolecules, 9(327), 1–17. Read more >