Porcine Insulin ELISA

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Minimal Number of Kits Required:

Porcine Insulin ELISA

(10-1200-01)

Kit format: 1 x 96 wells

Measurement range: 2.3 - 173 mU/L

Specially optimized for porcine samples

Highly specific, no cross-reactivity to proinsulin or C-peptide

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Description

Mercodia Porcine Insulin ELISA

A species-optimized, high quality immunoassay for the quantification of porcine insulin in serum or plasma.

Mercodia Animal Insulin Control (10-1221 -01) is designed to be used as a three-level control for Porcine Insulin ELISA.

Test characteristics

Species

Porcine

Sample types

Serum and plasma

Sample volume

25 µL

Assay range

2.3 - 173 mU/L

Detection limit

≤1.15 mU/L

Incubation

2h + 15 min

Detection method

Absorbance

specificity

The following cross-reactions have been tested:

Porcine C-peptide

< 0.001 %

Porcine proinsulin

< 0.2 %

Equine insulin

100 %

Canine insulin

100 %

Human insulin

108 %

Human C-peptide

< 0.01 %

Human proinsulin

< 0.1 %

NovoRapid

3.2 %

Levemir

Not detected

Lantus

27 %

Humalog

Not detected

directions for use Certificate of Analysis

directions for use

LOT. NO.	DOCUMENT
36803	DfU - 10-1200-01 v 10.0
36513	DfU - 10-1200-01 v 10.0
35698	DfU - 10-1200-01 v 10.0
35453	DfU - 10-1200-01 v 10.0
32443	DfU - 10-1200-01 v 10.0

Certificate of Analysis

LOT. NO.	DOCUMENT
36803	CoA - 10-1200-01
36513	CoA - 10-1200-01
35698	CoA - 10-1200-01

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references

Kemter, E. et al. (2017). INS-eGFP transgenic pigs: a novel reporter system for studying maturation, growth and vascularisation of neonatal islet-like cell clusters. Diabetologia, Vol. 60, pp. 1152–1156. Read more >

Tian, H. et al. (2018). Uniform Core–Shell Nanoparticles with Thiolated Hyaluronic Acid Coating to Enhance Oral Delivery of Insulin. Advanced Healthcare Materials, 7(17), 1800285. Read more >

Seibert, J. T. et al. (2018). Effects of heat stress and insulin sensitizers on pig adipose tissue. Journal of Animal Science, 96(2), 510–520. Read more >