Equine Insulin ELISA

(10-1205-01)

Kit format: 1 x 96 wells

Measurement range: 2.3 - 173 mU/L

Specially optimized for equine samples

Useful tool for laminitis investigations

Controls available: 10-1221-01

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Description

The Mercodia Equine Insulin ELISA is a species-optimized, high quality enzyme immunoassay for the quantification of equine insulin in serum or plasma.

Test characteristics

Species

Equine

Sample types

Serum, EDTA, heparin and citrate plasma

Sample volume

25 µL

Assay range

2.3 - 173 mU/L

Detection limit

1.15 mU/L

Incubation

2 h + 15 min

Detection method

Absorbance

specificity

The following cross-reactions have been tested:

Porcine Insulin

100 %

Porcine C-peptide

< 0.001 %

Porcine Proinsulin

< 0.2 %

Human Insulin

108 %

Human C-peptide

< 0.01 %

Human Proinsulin

< 0.1 %

NovoRapid

3.2 %

Levemir

Not detected

Lantus

27 %

Humalog

Not detected

directions for use & Safety Data Sheet

Directions for Use - 10-1205-01 Version 8.0 Lot.no 30096, 31916, 32442, 35179, 35521

Safety Data Sheet - 10-1205-01

For other languages and older versions:

Technical Library

references

Lindåse, S. et al. (2016). A modified oral sugar test for evaluation of insulin and glucose dynamics in horses. Acta Veterinaria Scandinavica, 58(S1), 64. Read more >

Kheder, M. H., Sillence, M. N., Bryant, L. M., & de Laat, M. A. (2017). The equine glucose-dependent insulinotropic polypeptide receptor: A potential therapeutic target for insulin dysregulation. Journal of Animal Science, 95(6), 2509–2516. Read more >

Kheder, M. H., Bailey, S. R., Dudley, K. J., Sillence, M. N., & de Laat, M. A. (2018). Equine glucagon-like peptide-1 receptor physiology. PeerJ, 2018(1), e4316. Read more >

Marycz, K., Kornicka, K., Szlapka-Kosarzewska, J., & Weiss, C. (2018). Excessive endoplasmic reticulum stress correlates with impaired mitochondrial dynamics, mitophagy and apoptosis, in liver and adipose tissue, but not in muscles in EMS horses. International Journal of Molecular Sciences, 19(1), 165. Read more >