Porcine C-Peptide ELISA

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Minimal Number of Kits Required:

Porcine C-Peptide ELISA

(10-1256-01)

Kit format: 1 x 96 wells

Measurement range: 20-1200 pmol/L

No cross reactivity to human proinsulin or insulin

No cross reactivity to C-peptide in human, macaque, rat or mouse

High specificity - enables specific measurement of porcine C-peptide in xenotransplantation studies

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Description

The Porcine C-peptide ELISA is an assay for the quantitative determination of porcine C-peptide in serum, plasma and cell culture medium.

The Porcine C-peptide ELISA may be used for post-transplant assessment of porcine islet xenograft function in human, macaque, rat or mouse. Enables distinguishing endogenously produced C-peptide for pharmacodynamic and/or pharmacokinetic studies during exogenous insulin administration

Test characteristics

Species

Porcine

Sample types

Serum, plasma and cell culture medium

Sample volume

10 µL

Assay range

20-1200 pmol/L

Detection limit

≤10 pmol/L

Incubation

2 h + 1 h + 15 min

Detection method

Absorbance

specificity

The following cross-reactions have been tested:

Porcine insulin

<0.05 %

Porcine proinsulin

<0.02 %

Human C-peptide

<0.003 %

Human insulin

<0.002 %

Human proinsulin

<0.01 %

Macaque C-peptide

<0.006 %

Mouse insulin

<0.13 %

Mouse proinsulin

<0.002 %

Rat C-peptide

0.08 %

Rat insulin

<0.002 %

Rat proinsulin

<0.002 %

directions for use Certificate of Analysis

directions for use

LOT. NO.	DOCUMENT
37092	DfU - 10-1256-01 v. 7.0
36731	DfU - 10-1256-01 v. 7.0
36649	DfU - 10-1256-01 v. 7.0
35461	DfU - 10-1256-01 v. 7.0
35651	DfU - 10-1256-01 v. 7.0
31419	DfU - 10-1256-01 v. 6.0

Certificate of Analysis

LOT. NO.	DOCUMENT
37092	CoA - 1256-01
36731	CoA - 10-1256-01
36649	CoA - 10-1256-01
35651	CoA -10-1256-01
31419	CoA -10-1256-01
35461	CoA -10-1256-01

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references

Manell, E. A. K., Rydén, A., Hedenqvist, P., Jacobson, M., & Jensen-Waern, M. (2014). Insulin treatment of streptozotocin-induced diabetes re-establishes the patterns in carbohydrate, fat and amino acid metabolisms in growing pigs. Laboratory Animals, 48(3), 261–269. Read more >

Ludwig, B. et al. (2017). Favorable outcome of experimental islet xenotransplantation without immunosuppression in a nonhuman primate model of diabetes. Proceedings of the National Academy of Sciences of the United States of America, 114(44), 11745–11750. Read more >

Lee, S. et al. (2019) Enhanced insulin production and reprogramming efficiency of mesenchymal stem cells derived from porcine pancreas using suitable induction medium. Xenotransplantation, 26(1) p. e12451.' Read more >