Rat/Mouse Proinsulin ELISA

Rat/Mouse Proinsulin ELISA

(10-1232-01)

Kit format: 1 x 96 wells

Measurement range: 3.5 - 200 pmol/L

Specific for rat and mouse proinsulin I and II

No cross reactivity to insulin or C-peptide

Fast and easy-to-use

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Description

Mercodia Rat/Mouse Proinsulin ELISA

The Rat/Mouse Proinsulin ELISA is a method for the quantitative determination of proinsulin in rat and mouse serum, plasma, cell culture medium and cellular extracts.

Test characteristics
Species

Rat and Mouse

Sample types

Serum, plasma, cell culture medium, tissue homogenate

Sample volume

25 µL

Assay range

3.5 - 200 pmol/L

Detection limit

≤3 pmol/L

Incubation

2 h+ 30 min

Detection method

Absorbance

specificity

The following cross-reactions have been tested:

Rat proinsulin I

100%

Rat proinsulin II

145 %

Rat insulin

Not detected

Rat C-peptide

Not detected

Mouse insulin

Not detected

Mouse C-peptide

Not detected

Mouse proinsulin I

234 %

Mouse proinsulin II

100 %

Human insulin

Not detected

Human C-peptide

Not detected

Human proinsulin

0.5 %

directions for use Certificate of Analysis Safety Data Sheet
directions for use
LOT. NO. DOCUMENT
35591 DfU - 10-1232-01 v. 11.0
Certificate of Analysis
LOT. NO. DOCUMENT
35591 CoA - 35591
Safety Data Sheet
DOCUMENT
SDS - 10-1232-01

For other languages and previous versions: Technical Library

references

Mehta, Z. B. et al. (2016). Changes in the expression of the type 2 diabetes-associated gene VPS13C in the β-cell are associated with glucose intolerance in humans and mice. American Journal of Physiology - Endocrinology and Metabolism, 311(2), E488–E507. Read more >

Shyr, Z. A., Wang, Z., York, N. W., Nichols, C. G., & Remedi, M. S. (2019). The role of membrane excitability in pancreatic β-cell glucotoxicity. Scientific Reports, 9(1), 6952. Read more >

Gilmore, R. et al. (2019). PDIA1/P4HB is required for efficient proinsulin maturation and ß cell health in response to diet induced obesity. ELIFE, 8, e44528 Read more >

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Rat/Mouse Proinsulin ELISA

(10-1232-01)

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Andreas Almlén

Key Account Manager Distributors and OEM

(+46) 70 780 95 09 BOOK A MEETING