IAPP ELISA

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IAPP ELISA

(10-1352-01)

Kit format: 1 x 96 wells

High sensitivity for measurement of low circulating IAPP concentrations

Analytically characterized for exploratory research use

On-demand* product. Specifications, availability, and lead times may vary.

Research Use Only (RUO)

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Description

The Mercodia On-demand* IAPP ELISA  provides a method for the quantitative determination of IAPP (islet amyloid polypeptide)/amylin in human plasma samples.

 

Mercodia On‑demand IAPP ELISA is a two‑site sandwich immunoassay using monoclonal antibodies specific for distinct epitopes on IAPP.  IAPP in the sample is captured by immobilized antibodies and detected using a peroxidase-conjugated antibody. Signal is generated using a chemiluminescent substrate and measured with a luminescence plate reader.

 

The ELISA detects mature IAPP and has demonstrated detection of the processing intermediate proIAPP(1-48), supporting a broader assessment of circulating IAPP. Additional characterization can further clarify the relative reactivity of this variant and other proIAPP intermediates.

 

The assay is analytically characterized for exploratory research use. See Directions for Use (DfU) and poster under related material for more information about the ELISA, and read more about IAPP here.

 

*Mercodia On‑demand products are not yet part of the standard portfolio and are intended for research applications where flexibility and access are prioritized over full portfolio‑level standardization. Validation scope and product characteristics are defined on a per‑product basis. Product specifications, availability, and lead times may vary. Contact us for more information. 

Test characteristics
Species

Human

Samples

Plasma

Sample volume

50 µL

Assay range

0.412 - 100 pM

Limit of detection (LOD)

0.197 pM

Incubation

1 h + 1 h + 15 min

Detection method

Chemiluminescence

specificity

The following cross-reactions have been tested:

Mouse IAPP

<0.01

CGRP-1

<0.01

CGRP-2

0.01

GLP-1

0.01

Glucagon

0.02

Insulin

0.01

directions for use Certificate of Analysis
directions for use
LOT. NO. DOCUMENT
Lj1352-137 DfU - 10-1352-01 v.1.0
Certificate of Analysis
LOT. NO. DOCUMENT
Lj1352-137 CoA - 10-1352-01
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Robert Almstedt

Customer Success Manager & Area Sales Manager Nordics

(+46) 72 292 15 08

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Enpack On demand 2

IAPP ELISA

(10-1352-01)

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Sylveniusgatan 8A,
SE-754 50
Uppsala, Sweden

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Robert Almstedt

Customer Success Manager & Area Sales Manager Nordics

(+46) 72 292 15 08

Contact Me

"*" indicates required fields

This field is for validation purposes and should be left unchanged.