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FREQUENTLY ASKED QUESTIONS

Access concise answers to frequently asked questions regarding our products to support your research needs.

FREQUENTLY ASKED QUESTIONS

The CoA for each product is available on its respective product page.

On each product page you can find the latest version of the DfU in English. If you need other versions of DfU, please send us your request by filling out the form in our Technical Library.

On each product page you can find the latest version of the SDS in English. If you need other versions of the SDS, please send us your request by filling out the form in our Technical Library.

You can find all technical notes in our Technical Library.

Plate sealers should not be used if not specifically stated in the Directions for Use. Sealing the plate may have a negative effect on the results if the assay has been designed and developed without the use of plate sealers. If the liquid in the wells is splashing during shaking of the plates, you need to check the settings of your shaker.

Plate sealers are included in all Mercodia kits where they should be used according to the Directions for Use, such as Mercodia Glucagon ELISA and Total GIP NL-ELISA.

The kit’s expiry date is listed on the label and in the Certificate of Analysis (CoA), which is available for download on each product page.

The sample reaction in Mercodia assays might be affected by pH and salt concentration. To ensure a proper reaction environment, sample buffer or Calibrator 0 included in the Mercodia ELISA kit should be used to dilute serum/plasma samples, antigen extraction or antigen in growth medium. Further information about dilution of samples is stated in the Directions for Use of each product. PBS or other salt solution is not recommended for sample dilution.

For several of the Mercodia ELISAs, which include all insulin assays, all proinsulin assays and Rat C-peptide ELISA, customers may separately order Diabetes Sample Buffer (10-1195-01) that can be used for diluting samples.

The validated sample types are stated in the Directions for Use (DfU).

For sample types other than those recommended in the DfU, a test to evaluate sample type and matrix compatibility can be performed to ensure reliable results. See technical note TN34-0123 in our Technical Notes page.

Calibrator 0 should not be used for blank subtraction, but merely be regarded as a negative control.

As stated in the Directions for Use of each product, a Mercodia ELISA is validated using Magellan software (Tecan) for absorbance-based ELISA, or MARS (BMG Labtech). You should be able to use similar sofwares to do your calculations.

Alternatively, it is also possible to perform curve-fitting, including both Five Parameter Logistic (5PL) and cubic spline, as well as data interpolation in commercial statistical software such as GraphPad Prism, or websites such as MyAssays.

When using website such as MyAssays, add the negative control (calibrator 0) equal to zero for the blank in the plate layout. The reason is that a blank is obligatory to add in the plate layout for the website, and Mercodia’s ELISA is not validated with blank reduction. For further information regarding how to use MyAssays, please see technical note TN34-0167 in our Technical Notes page.

You should never extrapolate the curve to values outside of the range of the calibrators. Instead assign the concentrations <Cal 1 or >Cal5.

You are always welcome to reach out to us at support@mercodia.com but you can also find helpful troubleshooting tips in our Troubleshooting page.

Within 2 days from the receipt of your order confirmation is our general rule. However, we do not ship within Europe on Fridays.

The plates used in the Mercodia assays are flat bottomed Nunc 96 plates produced by Thermo Fischer.

We strive to get back to you with an order confirmation within 24 hours of receiving your order. It could take longer depending on the current workload but feel free to send us a new email after 24 hours to double-check.

Yes, the products will meet specifications even if the package is delayed for several weeks. Our products are shipped in ambient temperature worldwide and have been stress and stability tested to make sure this is safe. Please see our Stability Statement here.

 

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Robert Almstedt

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Robert Almstedt

Area Sales Manager Denmark, Iceland, Norway and Sweden

(+46) 72 292 15 08 BOOK A MEETING